pRB and pRB-interacting TFs associate with the NOL7 promoter. (A) NOL7 promoter schematic depicting predicted SP1 and E2F binding sites. (B–E) ChIP analysis to evaluate SP1, E2F, pRB, and ppRB association on the NOL7 promoter in HeLa, HaCaT, and Hec-1A cell lines as described previously. (F) Western blot analysis depicting expression of TFs in these cell lines.