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Time course of the protective effect of the Pi infusion on astrocytes. Astrocytes were preincubated with the Pi infusion (100 μg/mL) for 2 h. H2O2 was then added and cytotoxicity was measured at the indicated time points. The results are the means ± SEM of four experiments (n = 16). **P < 0.01, ***P < 0.001, compared to cells treated with H2O2 only.
