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Preincubation of astrocytes with Pi infusion is a prerequisite for the protective effect against H2O2 cytotoxicity. Pi infusion (12.5 μg/mL) was added to astrocytes before (−2 h, −1 h) or after (1 h, 2 h) the addition of H2O2. Cytotoxicity was measured 20 h later. The results are means ± SEM of one experiment (n = 4) out of three independent experiments. **P < 0.001 *P < 0.01.
