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. Author manuscript; available in PMC: 2013 Apr 18.
Published in final edited form as: Cell Host Microbe. 2012 Oct 18;12(4):598–604. doi: 10.1016/j.chom.2012.09.005

Figure 3. Loop L4 is a functional module of MxA antiviral activity.

Figure 3

A. Human L4 rescues mouse Mx1 antiviral activity against the THOV minireplicon. mmMx1 expression constructs were co-transfected with the THOV minireplicon system into 293T cells; the experiment was otherwise carried out as in Figure 2B. mmMx1(hsL4) contains an exchange of mmL4 for the L4 of hsMxA. mmMx1(K49A) is a GTPase inactive mutant with no antiviral activity.

B. Human L4 determines the antiviral activity of mmMx1 against THOV infection. Percent infectivity was measured as described in Figure 2C. Error bars represent standard deviation of three biological replicates. ***, p<0.0001.

C. L4 mediates the interaction between MxA and THOV NP. Co-immunoprecipitation of viral NP with hsMxA and mmMx1 proteins was performed with lysates of THOV-infected (+) or uninfected (−) 293T cells transfected with the indicated Flag-tagged Mx expression constructs. Immunoprecipitation was performed using anti-Flag-affinity gel and the precipitated proteins were detected by Western blot with viral NP and MxA-specific antibodies. The lower panel shows the input of viral NP, MxA and β-tubulin in whole cell lysates. See also Figure S3.