Skip to main content
. Author manuscript; available in PMC: 2014 Jan 8.
Published in final edited form as: Biochemistry. 2012 Dec 26;52(1):221–227. doi: 10.1021/bi3014455

Figure 6.

Figure 6

Effects of bicyclopentadione on topoisomerase II-mediated DNA cleavage. The effects of bicyclopentadione (structure shown in Figure 1) in the absence (open circles, – Oxidant) or presence (closed circles, + Oxidant) of 50 μM K3Fe(CN)6 on the cleavage of negatively supercoiled plasmid DNA by human topoisomerase IIα were determined. Inset: curcumin was incubated in the absence (−) or presence (+) of K3Fe(CN)6 for 10 min before addition to topoisomerase IIα-DNA cleavage assay mixtures that contained 50 μM K3Fe(CN)6. Error bars represent standard deviations for three independent experiments.