. Author manuscript; available in PMC: 2013 Dec 15.

Published in final edited form as: Transplantation. 2012 Dec 15;94(11):10.1097/TP.0b013e3182751efd. doi: 10.1097/TP.0b013e3182751efd

Table 3.

MiRNA sequence variations in discovery cohort biopsies

		Position					IFTA libraries^c			Normal libraries^c

MiRNA precursor	Region	Genomic coordinates	Pos.^a	Pos.^b	Change	Comment	M1	M3	M4	M5	M6	M7	M8

mir-376c	mature	14:101506074:+	53	6	A>G	editing^d	ND	0.722	0.840	0.667	0.619	0.471	0
mir-376a-1	mature	14:101507167:+	53	6	A>G	editing^d	ND	ND	0.958	1.000	1.000	ND	ND
mir-376a-2	mature	14:101506460:+	53	6	A>G	editing^d	ND	ND	0.958	1.000	1.000	ND	ND
mir-215	mature	1:220291259: −	30	20	A>C		ND	0.615	ND	0.766	ND	0	0
mir-196a-2	star	12:54385599:+	64	18	C>T	rs11614913	ND	ND	ND	0.909	ND	ND	ND
mir-143	3p	5:148808562:+	66	1	A>T		0.346	0.345	0.336	0.338	0.365	0.332	0.347
mir-151	3p	8:141742684: −	69	2	C>A		0.579	0	0.579	0.618	0.739	0.588	0.524
mir-92a-1	3p	13:92003637:+	70	1	T>A		ND	0.611	0.490	0.469	0.431	0.518	0.608
mir-24-1	3p	9:97848368:+	71	1	G>T		0.556	0	0.519	0.469	0.522	0.485	0.568
mir-378	3p	5:149112452:+	71	1	C>A		0	0.536	0.481	0.589	0.619	0.727	0.579
mir-92a-2	3p	X:133303573: −	72	1	G>A		ND	0.786	0.714	0.852	0.920	0.878	0.835
mir-199a-2	3p	1:172113693: −	72	2	G>A		0	0	0	0	0.442	0.488	0.415
mir-24-2	3p	19:13947102: −	71	1	G>T		0	0	0	0	0.364	0.392	0.394
mir-423	3p	17:28444172:+	70	1	C>A		0	0	0	0.500	0.706	0	0
mir-324	3p	17:7126626: −	68	1	G>T		ND	ND	ND	0	0	ND	0.667
mir-125b-2	3p	21:17962634:+	72	1	A>T		ND	ND	0	0	0.684	0	0
mir-148b	3p	12:54731084:+	72	1	C>T		0	0	0	0	0	0	0
mir-27a	3p	19:13947260: −	73	1	C>A		0	0	0	0	0.336	0	0
let-7c	loop	21:17912180:+	33	1	T>A		0.278	0.245	0.203	0.149	0.158	0.164	0.149
let-7i	loop	12:62997493:+	33	1	G>A		0.410	0.356	0.346	0.376	0.376	0.298	0.314
mir-143	loop	5:148808528:+	32	1	T>A		0	0.202	0.206	0.274	0.245	0.410	0.307
mir-26a-2	loop	12:58218440: −	33	1	G>T		0	0.383	0.253	0.365	0.403	0.300	0.225
mir-26a-1	loop	3:38010926:+	33	1	G>T		0	0	0.242	0.360	0.366	0.254	0.211
mir-125b-1	loop	11:121970516: −	33	1	T>A		ND	0.920	0.571	0.698	0.576	0	0.627
mir-125b-2	loop	21:17962595:+	33	1	G>A		ND	1.000	0.690	0.909	0.704	0	0.914
mir-194-1	loop	1:220291547: −	33	1	C>A		ND	0	ND	0.738	ND	0.786	0
mir-26a-1	loop	3:38010926:+	33	1	G>A		0	0	0	0.208	0	0.261	0.154
mir-99a	loop	21:17911443:+	33	2	G>A		0	0	0	0.368	0.212	0	0
let-7b	loop	22:46509594:+	34	2	C>T		0	0	0	0.295	0	0	0.180
mir-143	loop	5:148808538:+	42	11	G>A		ND	ND	0	0	0.526	ND	ND
mir-100	loop	11:122022982: −	33	1	G>A		0	0	0	0	0	0	0
mir-200b	loop	1:1102537:+	44	12	C>A		ND	ND	ND	ND	ND	1.000	ND
mir-21	5p	17:57918633:+	10	10	G>C		ND	0	0.475	0	0.417	0	0.396

33 distinct nucleotide variations were identified, 5 of which were located in mature/star miRNA sequences, 1 in a 5′ region, 14 in loops and 13 in the 3′ regions of a precursor.

Position in the entire precursor follows our previously published definitions (5).

Position within the specific sub-region of the precursor follows our previously published definitions (5).

Numbers denote the ratio of varied divided by total reads at the indicated position; ND denotes sequence coverage insufficient to analyze (< 10 reads with the respective position)

A->G changes in mature sequence of miR-376a-1, mir-376a-2 and mir-376c are likely representing A to I RNA editing by dsRNA-specific adenosine deaminase. This specific editing event and its effect on targeting have been previously described (21, 22), and is supported by a well-represented unimodal distribution of the nucleotide variation frequency from a previous study (5).