Table 3.
Marker | Chr. | Repeat | Forward primer 5′–3′
|
Label | uM | PE (min–max) (breeds) | PE (min–max) (random) |
---|---|---|---|---|---|---|---|
Reverse primer 5′–3′ | |||||||
FCA069 | B4 | AC | AATCACTCATGCACGAATGC | VIC | 0.20 | 0.1324–0.5336 | 0.3958–0.5948 |
AATTTAACGTTAGGCTTTTTGCC | |||||||
FCA075 | E2 | TG | ATGCTAATCAGTGGCATTTGG | NED | 0.10 | 0.1442–0.5771 | 0.4240–0.5992 |
GAACAAAAATTCCAGACGTGC | |||||||
FCA105 | A2 | TG | TTGACCCTCATACCTTCTTTGG | PET | 0.20 | 0.2221–0.5585 | 0.6110–0.7101 |
TGGGAGAATAAATTTGCAAAGC | |||||||
FCA149a | B1 | TG | CCTATCAAAGTTCTCACCAAATCA | PET | 0.18 | 0.1783–0.5995 | 0.3586–0.5767 |
GTCTCACCATGTGTGGGATG | |||||||
FCA220 | F2 | CA | CGATGGAAATTGTATCCATGG | FAM | 0.30 | 0.0000–0.3383 | 0.1851–0.4221 |
GAATGAAGGCAGTCACAAACTG | |||||||
FCA229 | A1 | GT | CAAACTGACAAGCTTAGAGGGC | NED | 0.25 | 0.0452–0.5131 | 0.3927–0.5813 |
GCAGAAGTCCAATCTCAAAGTC | |||||||
FCA310a | C2 | (CA)5TA(CA)7
TA(CA)8 |
TTAATTGTATCCCAAGTGGTCA | FAM | 0.30 | 0.1196–0.5256 | 0.3417–0.5611 |
TAATGCTGCAATGTAGGGCA | |||||||
FCA441b | D3 | TAGA | ATCGGTAGGTAGGTAGATATAG | VIC | 0.15 | 0.2061–0.5774 | 0.3388–0.5505 |
GCTTGCTTCAAAATTTTCAC | |||||||
FCA678d | A1 | AC | TCCCTCAGCAATCTCCAGAA | NED | 0.25 | 0.0415–0.4908 | 0.3016–0.5715 |
GAGGGAGCTAGCTGAAATTGTT | |||||||
AMELc | XY | – | CGAGGTAATTTTTCTGTTTACT | N/A | N/A | ||
GAAACTGAGTCAGAGAGGC | |||||||
ZFXYc | XY | – | AAGTTTACACAACCACCTGG | PET | 0.20 | N/A | N/A |
CACAGAATTTACACTTGTGCA | |||||||
Total PE | 0.9008–0.9979 | 0.9947–0.9987 |
Markers that are of the first ten published feline microsatellites [106].
A marker that is currently included in the feline forensic panel [107].
The two markers on the X and Y chromosomes were added to the panel after the comparison test [108].
Newly designed primers presented herein for FCA678 generate a product 30 bp less than originally published primers.