Table 3.

Genetic markers selected as a “core” panel for ISAG cat parentage and identification testing.

Marker	Chr.	Repeat	Forward primer 5′–3′	Label	uM	PE (min–max) (breeds)	PE (min–max) (random)
			Reverse primer 5′–3′
FCA069	B4	AC	AATCACTCATGCACGAATGC	VIC	0.20	0.1324–0.5336	0.3958–0.5948
			AATTTAACGTTAGGCTTTTTGCC
FCA075	E2	TG	ATGCTAATCAGTGGCATTTGG	NED	0.10	0.1442–0.5771	0.4240–0.5992
			GAACAAAAATTCCAGACGTGC
FCA105	A2	TG	TTGACCCTCATACCTTCTTTGG	PET	0.20	0.2221–0.5585	0.6110–0.7101
			TGGGAGAATAAATTTGCAAAGC
FCA149a	B1	TG	CCTATCAAAGTTCTCACCAAATCA	PET	0.18	0.1783–0.5995	0.3586–0.5767
			GTCTCACCATGTGTGGGATG
FCA220	F2	CA	CGATGGAAATTGTATCCATGG	FAM	0.30	0.0000–0.3383	0.1851–0.4221
			GAATGAAGGCAGTCACAAACTG
FCA229	A1	GT	CAAACTGACAAGCTTAGAGGGC	NED	0.25	0.0452–0.5131	0.3927–0.5813
			GCAGAAGTCCAATCTCAAAGTC
FCA310a	C2	(CA)5TA(CA)7 TA(CA)8	TTAATTGTATCCCAAGTGGTCA	FAM	0.30	0.1196–0.5256	0.3417–0.5611
			TAATGCTGCAATGTAGGGCA
FCA441b	D3	TAGA	ATCGGTAGGTAGGTAGATATAG	VIC	0.15	0.2061–0.5774	0.3388–0.5505
			GCTTGCTTCAAAATTTTCAC
FCA678d	A1	AC	TCCCTCAGCAATCTCCAGAA	NED	0.25	0.0415–0.4908	0.3016–0.5715
			GAGGGAGCTAGCTGAAATTGTT
AMELc	XY	–	CGAGGTAATTTTTCTGTTTACT			N/A	N/A
			GAAACTGAGTCAGAGAGGC
ZFXYc	XY	–	AAGTTTACACAACCACCTGG	PET	0.20	N/A	N/A
			CACAGAATTTACACTTGTGCA
				Total PE		0.9008–0.9979	0.9947–0.9987

^aMarkers that are of the first ten published feline microsatellites [106].

^bA marker that is currently included in the feline forensic panel [107].

^cThe two markers on the X and Y chromosomes were added to the panel after the comparison test [108].

^dNewly designed primers presented herein for FCA678 generate a product 30 bp less than originally published primers.