Figure 8.

Delayed demyelination following diffuse ischemic stroke. A–D, Representative confocal images depicting MBP labeling in CD68- and GFAP-positive lesions 3 and 28 d after diffuse stroke injury. E, Changes in myelination were assessed by image analysis. Color channels were split, and the MBP channel was background subtracted, uniformly thresholded, and converted into a binary image. MBP-positive regions of interest (ROIs) were generated and the length calculated. The median ROI length was calculated and designated as an arbitrary value for “myelin continuity.” Myelin continuity was compared across time points by expressing the ratio between values measured in ipsilateral lesions versus mirror-image contralateral control tissue. F, Twenty-eight days after stroke, myelin continuity was significantly reduced compared to control values, reflecting delayed myelin degeneration. *p < 0.05 (ANOVA). Scale bars: 25 μm.