Figure 2.

Caryophyllene production in heterozygous deletion strains. (A) Expression of Sf126 orf in yeast yields trans-caryophyllene (peak 1) and minor amounts of α-humulene (peak 2) sampled by SPME and analyzed by GC-MS; (B) production of caryophyllene by diploid heterozygous deletion strains in BY4743 genetic background. BY4743 cells were used as control strains; (C) production of caryophyllene in diploid heterozygous deletion strains overexpressing stable variants of HMG2 (K6R) from extra gene copies integrated into the chromosomes.