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Increased stabilization of hmg1p. (A) Overlay of flow cytometry measurements of EG60 cells (black) and AM109 (red) overexpressing the transmembrane hmg1p domain fused to EYFP, hmg1-TM-EYFP, showing increased amounts of protein stability. Thirty thousand cells were counted; (B) Mean fluorescence values for strains analyzed show fluorescence increasing with each successive perturbation. (C) Hmg2-TM-EYFP fluorescing AM109 yeast cells; (D) Hmg1-TM-EYFP fluorescing AM109 yeast cells.
