Table 1.
List of S. cerevisiae strains used
| Strain | Genotype | Plasmid description | Source |
|---|---|---|---|
| BY4741 |
Mat a, his3Δ1, leu2Δ0, met15Δ0, ura3Δ0. |
|
Research Genetics |
| EG60 |
Mat α, ura3, trp1, his3. |
|
E. Golemis |
| EG60-01 |
Mat α, ura3, trp1, his3. |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AM62 |
Mat α, ura3, trp1, his3, ubc7:: HIS5 |
|
This study |
| AM62-01 |
Mat α, ura3, trp1, his3, ubc7:: HIS5 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AM66 |
Mat α/a, PGal1-HMG2(K6R)x2::HO, ura3, trp1, his3. Derivative of AM63 and KSY10 back-crossed 4 times. |
|
This study |
| AM85 |
Mat α/a, PGal1-HMG2(K6R)x2::HO, ura3, trp1, TDH3p-HMG2(K6R)-HIS3::leu2. Derivative of AM66 |
|
This study |
| AM88 |
Mat α, PGal1-HMG2(K6R)::HO, ura3, trp1, PTDH3-HMG2(K6R)-HIS3::leu2. Derived from sporulation of AM85 and subsequent excision of HIS3 |
|
This study |
| AM88-01 |
Mat α, PGal1-HMG2(K6R)::HO, ura3, trp1, PTDH3-HMG2(K6R)-HIS3::leu2. Derived from sporulation of AM85 and subsequent excision of HIS3 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AM89 |
Mat a, PGal1-HMG2(K6R)::HO, ura3, trp1, PTDH3-HMG2(K6R)-HIS3::leu2. Derived from sporulation of AM85 and subsequent excision of HIS3 |
|
This study |
| AM90 |
Mat a/α, PGal1-HMG2(K6R):: HOX2, ura3, trp1, his3, PTDH3-HMG2(K6R)::leu2 X2. Derived from cross of AM88 and AM89 |
|
This study |
| AM94 |
Mat a/α, PGal1-HMG2(K6R):: HOX2, ura3, trp1, his3, PTDH3-HMG2(K6R)::leu2 X2, ERG9/erg9 Derived from AM90 |
|
This study |
| AM94-01 |
Mat a/α, PGal1-HMG2(K6R):: HOX2, ura3, trp1, his3, PTDH3-HMG2(K6R)::leu2 X2, ERG9/erg9 Derived from AM90 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AM97 |
Mat a/α, PGal1-HMG2(K6R):: HOX2, ura3, trp1, his3, PTDH3-HMG2(K6R)::leu2 X2, ERG9/erg9, UBC7/ubc7 derived from AM94 |
|
This study |
| AM97-01 |
Mat a/α, PGal1-HMG2(K6R):: HOX2, ura3, trp1, his3, PTDH3-HMG2(K6R)X2-::leu2 ERG9/erg9, UBC7/ubc7 derived from AM94 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AM102 |
Mat a/α, PGal1-HMG2(K6R):: HOX2, ura3, trp1, his3, PTDH3-HMG2(K6R)X2-::leu2 ERG9/erg9, UBC7/ubc7, SSM4/ssm4 derived from AM97 |
|
This study |
| AM102-01 |
Mat a/α, PGal1-HMG2(K6R):: HOX2, ura3, trp1, his3, PTDH3-HMG2(K6R)::leu2 X2, ERG9/erg9, UBC7/ubc7, SSM4/ssm4 derived from AM97 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AM109 |
Mat a/α, PGal1-HMG2(K6R):: HOX2, ura3, trp1, his3, PTDH3-HMG2(K6R)X2-::leu2 ERG9/erg9, UBC7/ubc7, SSM4/ssm4, PHO86/pho86, derived from AM102 |
|
This study |
| AM109-01 |
Mat a/α, PGal1-HMG2(K6R)x2:: HOX2, ura3, trp1, his3, PTDH3-HMG2(K6R)X2-::leu2 ERG9/erg9, UBC7/ubc7, SSM4/ssm4, PHO86/pho86, derived from AM102 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMW2 |
Mat a/α, ura3, his3, leu2 TRP1/trp1, DCR2/dcr2::G418 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMW3 |
Mat a/α, ura3, his3, leu2 TRP1/trp1, SSD1/ssd1::G418 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMW4 |
Mat a/α, ura3, his3, leu2 TRP1/trp1, UBC7/ubc7::G418 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMW5 |
Mat a/α, ura3, his3, leu2 TRP1/trp1, UBI4/ubi4::G418 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMW6 |
Mat a/α, ura3, his3, leu2 TRP1/trp1, UBX2/ubx2::G418 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMW7 |
Mat a/α, ura3, his3, leu2 TRP1/trp1, DER1/der1::G418 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMW8 |
Mat a/α, ura3, his3, leu2 TRP1/trp1, SPF1/spf1::G418 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMW9 |
Mat a/α, ura3, his3, leu2 TRP1/trp1, NPL4/npl4::G418 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMW10 |
Mat a/α, ura3, his3, leu2 TRP1/trp1, SKM1/skm1::G418 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMW11 |
Mat a/α, ura3, his3, leu2 TRP1/trp1, CUE1/cue1::G418 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMW12 |
Mat a/α, ura3, his3, leu2 TRP1/trp1, SSM4/ssm4::G418 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMW13 |
Mat a/α, ura3, his3, leu2 TRP1/trp1, DOA1/doa1::G418 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMW14 |
Mat a/α, ura3, his3, leu2 TRP1/trp1, HMG1/hmg1::G418 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMW15 |
Mat a/α, ura3, his3, leu2 TRP1/trp1, CHO2/cho2::G418 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMW16 |
Mat a/α, ura3, his3, leu2 TRP1/trp1, GRE3/gre3::G418 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMW17 |
Mat a/α, ura3, his3, leu2 TRP1/trp1, WHI2/whi2::G418 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMW18 |
Mat a/α, ura3, his3, leu2 TRP1/trp1, YTA7/yta7::G418 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMW19 |
Mat a/α, ura3, his3, leu2 TRP1/trp1, PHO86/pho86::G418 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMW20 |
Mat a/α, ura3, his3, leu2 TRP1/trp1, RIC1/ric1::G418 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMW21 |
Mat a/α, ura3, his3, leu2 TRP1/trp1, GAS1/gas1::G418 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMW22 |
Mat a/α, ura3, his3, leu2 TRP1/trp1, ADH1/adh1::G418 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMW23 |
Mat a/α, ura3, his3, leu2 TRP1/trp1, GDH1/gdh1::G418 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMW24 |
Mat a/α, ura3, his3, leu2 TRP1/trp1, HRD1/hrd1::G418 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMW1 |
Mat a/α, ura3, his3, leu2 TRP1/trp1 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMW25 |
Mat a/α, ura3, his3, leu2 TRP1/trp1, HRD3/hrd3::G418 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| ΑΜΗ9 |
Mat a/α, PGal1-HMG2(K6R)::HO, ura3, his3, trp1/TRP1, PTDH3-HMG2(K6R)-HIS5::leu2 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMH13 |
Mat a/α, PGal1-HMG2(K6R)::HO, ura3, his3, trp1/TRP1, PTDH3-HMG2(K6R)-HIS5::leu2, DCR2/dcr2::G418 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMH15 |
Mat a/α, PGal1-HMG2(K6R)::HO, ura3, his3, trp1/TRP1, PTDH3-HMG2(K6R)-HIS5::leu2, SSD1/ssd1::G418 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMH16 |
Mat a/α, PGal1-HMG2(K6R)::HO, ura3, his3, trp1/TRP1, PTDH3-HMG2(K6R)-HIS5::leu2, UBC7/ubc7::G418 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMH17 |
Mat a/α, PGal1-HMG2(K6R)::HO, ura3, his3, trp1/TRP1, PTDH3-HMG2(K6R)-HIS5::leu2, UBI4/ubi4::G418 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMH18 |
Mat a/α, PGal1-HMG2(K6R)::HO, ura3, his3, trp1/TRP1, PTDH3-HMG2(K6R)-HIS5::leu2, UBX2/ubx2::G418 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMH19 |
Mat a/α, PGal1-HMG2(K6R)::HO, ura3, his3, trp1/TRP1, PTDH3-HMG2(K6R)-HIS5::leu2, DER1/der1::G418 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMH20 |
Mat a/α, PGal1-HMG2(K6R)::HO, ura3, his3, trp1/TRP1, PTDH3-HMG2(K6R)-HIS3::leu2, SPF1/spf1::G418 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMH21 |
Mat a/α, PGal1-HMG2(K6R)::HO, ura3, his3, trp1/TRP1, PTDH3-HMG2(K6R)-HIS5::leu2, NPL4/npl4::G418 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMH22 |
Mat a/α, PGal1-HMG2(K6R)::HO, ura3, his3, trp1/TRP1, PTDH3-HMG2(K6R)-HIS5::leu2, SKM1/skm1::G418 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMH23 |
Mat a/α, PGal1-HMG2(K6R)::HO, ura3, his3, trp1/TRP1, PTDH3-HMG2(K6R)-HIS5::leu2, CUE1/cue1::G418 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMH24 |
Mat a/α, PGal1-HMG2(K6R)::HO, ura3, his3, trp1/TRP1, PTDH3-HMG2(K6R)-HIS5::leu2, SSM4/ssm4::G418 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMH25 |
Mat a/α, PGal1-HMG2(K6R)::HO, ura3, his3, trp1/TRP1, PTDH3-HMG2(K6R)-HIS5::leu2, DOA1/doa1::G418 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMH26 |
Mat a/α, PGal1-HMG2(K6R)::HO, ura3, his3, trp1/TRP1, PTDH3-HMG2(K6R)-HIS5::leu2, HMG1/hmg1::G418 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMH27 |
Mat a/α, PGal1-HMG2(K6R)::HO, ura3, his3, trp1/TRP1, PTDH3-HMG2(K6R)-HIS3::leu2, CHO2/cho2::G418 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMH28 |
Mat a/α, PGal1-HMG2(K6R)::HO, ura3, his3, trp1/TRP1, PTDH3-HMG2(K6R)-HIS5::leu2, GRE3/gre3::G418 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMH29 |
Mat a/α, PGal1-HMG2(K6R)::HO, ura3, his3, trp1/TRP1, PTDH3-HMG2(K6R)-HIS5::leu2, WHI2/whi2::G418 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMH30 |
Mat a/α, PGal1-HMG2(K6R)::HO, ura3, his3, trp1/TRP1, PTDH3-HMG2(K6R)-HIS5::leu2, YTA7/yta7::G418 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMH31 |
Mat a/α, PGal1-HMG2(K6R)::HO, ura3, his3, trp1/TRP1, PTDH3-HMG2(K6R)-HIS5::leu2, PHO86/pho86::G418 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMH32 |
Mat a/α, PGal1-HMG2(K6R)::HO, ura3, his3, trp1/TRP1, PTDH3-HMG2(K6R)-HIS5::leu2, RIC1/ric1::G418 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMH33 |
Mat a/α, PGal1-HMG2(K6R)::HO, ura3, his3, trp1/TRP1, PTDH3-HMG2(K6R)-HIS5::leu2, GAS1/gas1::G418 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMH34 |
Mat a/α, PGal1-HMG2(K6R)::HO, ura3, his3, trp1/TRP1, PTDH3-HMG2(K6R)-HIS5::leu2, BFR1/bfr1::G418 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMH47 |
Mat a/α, ura3, his3, leu2 TRP1/trp1, DCR2/dcr2::G418, UBC7/ubc7:: HIS5 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMH49 |
Mat a/α, ura3, his3, leu2 TRP1/trp1, SSD1/ssd1::G418, UBC7/ubc7:: HIS5 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMH50 |
Mat a/α, ura3, his3, leu2 TRP1/trp1, ubc7::G418, ubc7:: HIS5 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMH51 |
Mat a/α, ura3, his3, leu2 TRP1/trp1, UBI4/ubi4::G418, UBC7/ubc7:: HIS5 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMH52 |
Mat a/α, ura3, his3, leu2 TRP1/trp1, UBX2/ubx2::G418, UBC7/ubc7:: HIS5 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMH53 |
Mat a/α, ura3, his3, leu2 TRP1/trp1, DER1/der1::G418, UBC7/ubc7:: HIS5 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMH54 |
Mat a/α, ura3, his3, leu2 TRP1/trp1, SPF1/spf1::G418, UBC7/ubc7:: HIS5 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMH55 |
Mat a/α, ura3, his3, leu2 TRP1/trp1, NPL4/npl4::G418,UBC7/ubc7:: HIS5 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMH56 |
Mat a/α, ura3, his3, leu2 TRP1/trp1, SKM1/skm1::G418, UBC7/ubc7:: HIS5 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMH57 |
Mat a/α, ura3, his3, leu2 TRP1/trp1, CUE1/cue1::G418, UBC7/ubc7:: HIS5 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMH58 |
Mat a/α, ura3, his3, leu2 TRP1/trp1, SSM4/ssm4::G418, UBC7/ubc7:: HIS5 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMH59 |
Mat a/α, ura3, his3, leu2 TRP1/trp1, DOA1/doa1::G418, UBC7/ubc7:: HIS5 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMH60 |
Mat a/α, ura3, his3, leu2 TRP1/trp1, HMG1/hmg1::G418, UBC7/ubc7:: HIS5 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMH61 |
Mat a/α, ura3, his3, leu2 TRP1/trp1, CHO2/cho2::G418, UBC7/ubc7:: HIS5 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMH62 |
Mat a/α, ura3, his3, leu2 TRP1/trp1, GRE3/gre3::G418, UBC7/ubc7:: HIS5 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMH63 |
Mat a/α, ura3, his3, leu2 TRP1/trp1, WHI2/whi2::G418, UBC7/ubc7:: HIS5 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMH64 |
Mat a/α, ura3, his3, leu2 TRP1/trp1, YTA7/yta7::G418, UBC7/ubc7:: HIS5 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMH65 |
Mat a/α, ura3, his3, leu2 TRP1/trp1, PHO86/pho86::G418, UBC7/ubc7:: HIS5 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMH66 |
Mat a/α, ura3, his3, leu2 TRP1/trp1, RIC1/ric1::G418, UBC7/ubc7:: HIS5 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMH69 |
Mat a/α, ura3, his3, leu2 TRP1/trp1, ADH1/adh1::G418, UBC7/ubc7:: HIS5 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMH70 |
Mat a/α, ura3, his3, leu2 TRP1/trp1, GDH1/gdh1::G418, UBC7/ubc7:: HIS5 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMH74 |
Mat a/α, ura3, his3, leu2 TRP1/trp1, HRD1/hrd1::G418, UBC7/ubc7:: HIS5 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMH76 |
Mat a/α, ura3, his3, leu2 TRP1/trp1 UBC7/ubc7:: HIS5 Control Strain |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMH77 |
Mat a/α, ura3, his3, leu2 TRP1/trp1 HRD3/hrd3::G418, UBC7/ubc7:: HIS5 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMH78 |
Mat a/alpha, ura3, his3, leu2 TRP1/trp1 BFR1/bfr1::G418, UBC7/ubc7:: HIS5 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMD21 |
Mat α, ura3, his3, leu2, pho86::G418, ubc7:: HIS5 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMD14 |
Mat α, ura3, his3, leu2, ssm4::G418, ubc7:: HIS5 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMD5 |
Mat α, ura3, his3, leu2, ssd1::G418, ubc7:: HIS5 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMD7 |
Mat α, ura3, his3, leu2, ubi4::G418, ubc7:: HIS5 |
pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 |
This study |
| AMD33 | Mat α, ura3, his3, leu2, hrd3::G418, ubc7:: HIS5 | pUTDH3/Sf126, 2 μ URA3 PTDH3-Sf126 | This study |