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. 2012 Dec 22;11:162. doi: 10.1186/1475-2859-11-162

Table 2.

List of oligonucleotide primers used

Primer name Sequence
5’TDH3p(HindIII)
5’-aagcttcagttcgagtttatcattatcaatactgcc-3’
3’TDH3p(BamHI)
5’- ggatccgtgtgtttattcgaaactaagttcttgg-3’
5’Sf126
5’-atggatattcctgtgattgttacttccgtttcg-3’
3’Sf126 XhoI
5’-ctcgagctagcaatcaagcatgaaggggtc-3’
5’GS linker
5’-gatcctatgtcgacggtagcggcagcggtagcggtagcggcagcgaattctatc-3’
3’GS linker
5’-tcgagatagaattcgctgccgctaccgctaccgctgccgctaccgtcgacatag-3’
5’Sf126
5’-atggatattcctgtgattgttacttccgtttcg-3’
HMG1-BamHI
5’-ggatccatgccgccgctattcaagggactgaaacagatggcaaa-3’
HMG1-TM-R-SalInS
5’-gtcgacgtcataatttttatatggtaaacgatcagatgctaatacagg-3’
5HMG2 EcoRI
5’-gaattcatgtcacttcccttaaaaacgatagtacat-3’
3HMG2-TM-XhoI
5’-ctcgagataatcataatttctgaagggcaa-3’
5’INT1-COD7
5’-gtttgccggtggtgcgaacaatagagcgaccatgaccttgaaggtgagacgcagttcgagtttatcattatc-3’
3’INT1-COD7
5’-ccagtgttgtatgtacctgtctatttatactggtagcaaccctggatctgatatcacctaataact-3’
ERG9-pUG F
5’-agagaaaagacgaagagcagaagcggaaaacgtatacacgtcacatatcacagctgaagcttcgtacgc-3’
ERG9-pUG R
5’-gtacttagttattgttcggagttgtttgtttatgttatttggcgcagactgcataggccactagtggatctg-3’
ERG9prom
5’-ctaaacgagcagcgagaacacgaccac-3’
UBC7 pUG F
5’-gagattatcctaaaaggaacttccctagtaatagtgtaatttggaagggcatagccagctgaagcttcgtacgc-3’
UBC7 pUG R
5’-gtatatagagaacagttaaaaggaagaccaaatgatcattaacctgctacctgctttcagcataggccactagtggatctg-3’
UBC7prom
5’-gaagaacttaccagactgtttcaagt-3’
SSM4-R
5’-tatatgtaaatatgctagcattcattttaaatgtaaggaagaaaacgcctgcataggccactagtggatctg-3’
SSM4-F
5’-tagccaagagtaccactaattgaatcaaagagactagaagtgtgaaagtccagctgaagcttcgtacgc-3’
SSM4prom
5’-gacattgaaaagatgattatgaccgatct-3’
PHO86-pUG F
5’-cgactcttccagttgcacattttttcatagcgaaagtacaagagtaggaacagctgaagcttcgtacgc-3’
PHO86-pUG R 5’-tttattttgttacttcctgcgagttgcaacagaactaacaagatgccatggcataggccactagtggatctg-3’