Table 1.
Bacterial strains and plasmids used in this work
| Strain/plasmid | Relevant characteristics | Source/reference |
|---|---|---|
|
Bacterial strains |
|
|
| NZ9000 |
A derivative of L. lactis MG1363 wild type strain generated by the integration of the NisRK genes |
45 |
| LL |
L. lactis MG1363 containing pOri23 plasmid |
40 |
| LL-mInlA+ |
L. lactis NZ9000 strain containing pOri253:mInlA |
This work |
| LL-BLG |
L. lactis MG1363 strain containing pOri23 and pValac: BLG plasmid |
32 |
| LLmInlA+BLG |
L. lactis NZ9000 strain expressing mInlA gene and carrying pValac: BLG plasmid |
This work |
|
Plasmids |
|
|
| pPL2:mInlA |
E. coli vector containing mInlA gene |
30 |
| pOri253Link |
L. lactis-E. coli shuttle vector, Eryr |
This work |
| pOri23 |
L. lactis-E. coli shuttle vector, Eryr |
40 |
| pValac: BLG |
L. lactis-E. coli shuttle vector carrying the BLG gene under the control of the eukaryotic promoter IE CMV, Cmr |
32 |
| pOri253:mInlA | L. lactis-E. coli shuttle vector carrying the mInlA gene under the control of the constitutive PrfA promoter protein and harboring the native cell wall anchoring signal | This work |
Eryr Erythromycin resistant; Cmr Chloramphenicol resistant.