Figure 3.
A trypsin- and EDTA-sensitive Plase A2 from bovine erythrocytes has higher activity against fluorescent PC than against PE and is inactive toward NAPE. (a) Bovine erythrocyte suspensions were incubated with C6-NBD analogs of PC, PE, or NAPE and degradation of the substrates was assessed as described in Materials and Methods. (Inset) Linear variation of assay results with erythrocyte concentration. (b) Degradation of fluorescent PC by bovine erythrocyte suspensions that received no treatment (control), were treated with trypsin, or assayed in the presence of 20 mM EDTA.