Table 2.
Repartition of enzymatic activities produced by T. xylanilyticus grown on various substrates
|
Xylanase (IU/mg)a |
Xylosidase (mIU/mg)a |
|||||
|---|---|---|---|---|---|---|
| Localization | Extracellular | Cytoplasmic | Membrane associated | Extracellular | Cytoplasmic | Membrane associated |
|
glucose |
nd |
nd |
nd |
2.2 ± 0.6 |
11.4 ± 3.8 |
nd |
|
xylan |
21.4 ± 1.6 |
4.7 ± 0.1 |
1.6 ± 0.1 |
4.7 ± 0.6 |
62.7 ± 5.0 |
nd |
|
wheat bran |
25.9 ± 3.5 |
4.9 ± 0.8 |
0.6 ± 0.0 |
3.1 ± 0.3 |
96.6 ± 2.5 |
nd |
|
wheat straw |
5.8 ± 0.3 |
1.7 ±0.2 |
0.5 ± 0.0 |
4.2 ± 0.3 |
18.9 ± 1.0 |
nd |
|
Feruloyl-esterase (mIU/mg)a |
Acetyl-esterase (mIU/mg)a |
|||||
|
Localization |
Extracellular |
Cytoplasmic |
Membrane associated |
Extracellular |
Cytoplasmic |
Membrane associated |
|
glucose |
12.0 ± 0.8 |
27.4 ± 1.0 |
1.5 ± 0.7 |
109.6 ± 4.6 |
174.7 ± 13.3 |
26.6 ± 1.9 |
|
xylan |
5.8 ± 0.6 |
23.9 ± 1.1 |
23.8 ± 2.9 |
76.2 ± 8.0 |
289.5 ± 13.0 |
nd |
|
wheat bran |
15.9 ± 2.2 |
14.3 ± 1.4 |
43.09 ± 0.7 |
64.2 ± 5.8 |
288.8 ± 35.3 |
nd |
|
wheat straw |
15.9 ± 2.7 |
146.4 ± 2.8 |
73.1 ± 4.3 |
130.0 ± 0.7 |
93.0 ± 14.5 |
nd |
|
Arabinofuranosidase (mIU/mg)a |
||||||
|
Localization |
Extracellular |
Cytoplasmic |
Membrane associated |
|||
|
glucose |
6.7 ± 0.3 |
32.2 ± 2.7 |
1.0 ± 0.0 |
|||
|
xylan |
16.0 ± 1.0 |
230.8 ± 2.2 |
23.9 ± 3.5 |
|||
|
wheat bran |
17.5 ± 0.2 |
399.1 ± 27.5 |
3.51 ± 0.6 |
|||
| wheat straw | 0.6 ± 0.0 | 64.3 ± 1.2 | 0.7 ± 0.1 | |||
a Activities are given in IU per mg of protein for xylanase and in mIU per mg of protein for arabinofuranosidase, feruloyl, acetyl-esterase and xylosidase activities nd: not detected.