Table 4.
Over-expression analyses
| WT + | Mean | N | SE | G1% | S/G2/M% | Budding index |
|---|---|---|---|---|---|---|
|
Empty Vector |
78 |
11 |
1 |
56.99 |
40.56 |
44.3 |
|
ECM9 |
62** |
11 |
1 |
52.21 |
47.79 |
60.3 |
|
CTR9 |
80 |
14 |
1 |
54 |
46 |
65.7 |
|
MRPL49 |
68** |
11 |
2 |
52.38 |
47.62 |
51.9 |
|
YJR114W |
68* |
13 |
2 |
72.53 |
27.47 |
44.4 |
|
RPL42A |
68** |
13 |
1 |
52.24 |
47.76 |
57.7 |
|
RPL16B |
72** |
13 |
1 |
44.31 |
55.69 |
62.3 |
|
CBS1 |
69* |
14 |
2 |
72.64 |
24.46 |
45.7 |
| RPL36B | 73 | 6 | 4 | 59.31 | 40.09 | 41.6 |
Over-expression analysis in wild type cells. This table illustrates the effects of over-expressing the new size mutant genes in wild type yeast cells. Data includes the average cell size from logarithmic cultures, number of replicates, standard error, % of cells in G1 and S/G2/M phases as determined by flow cytometry, and the budding index values. Statistical differences in mean cell size values were calculated using unpaired t-test (**p<0.0001, *p<0.0051). All analyses were carried out in -Ura Raf/Gal media using GAL-inducible vectors. Vector alone (pB63) was used as a control. Note: We were unable to generate a GAL-ROM2 inducible vector.