Skip to main content
. 2013 Jan 9;8(1):e53934. doi: 10.1371/journal.pone.0053934

Figure 1. Time course of the protocol.

Figure 1

Once the animal was connected to the ventilator, a recruitment maneuver (RM) was followed by impedance measurement (Z) and HCl treatment. After a stabilization period and several RM and Z, a 60 min ventilation period (thick arrow) was started. During the ventilation period, Z and peak airway pressure (PAP) were recorded at 5 min intervals. At the end of the protocol, blood gases and a lavage sample were obtained and the lung was isolated for further processing.