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. 2013 Jan 9;8(1):e53512. doi: 10.1371/journal.pone.0053512

Figure 5. BLRF2 rescues ORF52 null MHV68 replication, but the BLRF2-ARA mutant cannot.

Figure 5

(A) Complementation assay measuring MHV68 virion release by quantitative PCR into supernatants of 293T cells co-transfected with replication defective MVH68 ORF52 null BAC and empty vector, wild-type FLAG-BLRF2, or FLAG-BLRF2-ARA mutant. Viral DNA was quantified four days post-transfection and the results shown are representative of two independent experiments performed in triplicate. Western blot with anti-flag antibody shows BLRF2-WT and –ARA expression levels.