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. 2013 Jan 9;8(1):e53423. doi: 10.1371/journal.pone.0053423

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© 2013 Gray et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Micrographs of a fluo-4AM loaded preparation showing a smooth muscle bundle and an associated ICC-IM pre-EFS and during 0.5 Hz EFS. B. Intensity-time plot from the 2 regions of interest indicated in F, showing activity in a SMC and an ICC-IM pre-EFS and during EFS (0.5 Hz). The SMC was firing at a higher frequency than the ICC pre-EFS with apparently no correlation between the 2 cell types. Neurogenic stimulation evoked large-amplitude, simultaneously Ca²⁺-responses in both cells.