Figure 4.

KalSR^KO/KO anterior pituitary cultures showed elevated basal secretion of growth hormone and prolactin. Cultured anterior pituitary cells from male or female WT and KalSR^KO/KO mice were fed with basal medium followed by medium containing 2 mM BaCl₂ , basal medium and medium containing 1 μM PMA. Cell lysates (1.67% of the total) and media (16.7% of the total) were probed by immunoblotting for growth hormone and prolactin; representative blots are shown. Secretion rates were calculated by expressing the amount of hormone in the medium as a percentage of the total amount of hormone in the cells at the end of the experiment; for the BaCl₂ and PMA samples, the basal rate of secretion was subtracted from the total rate of secretion in the presence of secretagogue. Since no sex-specific differences in secretion rates were observed, data for male and female cultures were averaged. Basal growth hormone and prolactin secretion were increased in KalSR^KO/KO mice (growth hormone t(4)= 3.26, p<0.05; prolactin t(6)=4, p<0.05); error bars are S.E.M.