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. 2013 Jan 9;8(1):e53950. doi: 10.1371/journal.pone.0053950

Figure 2. Pathological examination of hearts from Wistar rats after TAAC surgery.

Figure 2

(A) Scanning images of the HE-stained paraffin sections of the hearts from Sham and TAAC rats, and the scale bar: 1 cm. (B) Microscopic images of the HE-stained paraffin sections of the hearts from Sham and TAAC rats, and the scale bar: 50 µm. Cardiac tissue color was lighter with inhomogeneous staining, numerous nuclear-free regions were evident, and an increased nucleolar density in muscle fibers in regions of atrophy was observed. (C) Microscopic images of the Masson’s-stained paraffin sections of the hearts from Sham and TAAC rats, and the scale bar: 50 µm. The regions of fibroplasia around blood vessels were presented as blue. (D)Heart weight index (HWI) was assessed by heart weight-to-body weight ratio. (E) The relative cross-sectional area was evaluated by calculating the ratio of the cardiac muscle fiber surface area of the microscopic images of the paraffin sections. Data are presented as means ± SEM. *P<0.05 compared with Sham.