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. 2013 Jan 9;8(1):e53950. doi: 10.1371/journal.pone.0053950

Figure 7. Autophagic vacuole number varies with beclin-1 gene expression.

Figure 7

The autophagic vacuoles was analyzed by transmission electron microscopy. (A) Representative autophagic vacuoles (arrows indicated) were detected by transmission electron microscopy. Magnification of the first seven images: ×13500. The final image showed an autophagic vacuole under x46000 magnificationor: part of the cytoplasm and the organelle were packaged into specific double-membrane structures. Scale bar of the first seven images: 1 µm. Scale bar of the final image:200 nm.1 µm. –regulation. (B) Evaluation of the influence of up-regulation of beclin-1 on autophagic vacuoles. Con: control group, Ang II: treated with 1 µmol/L Angiotensin II, miR-30a inhibitors: treated with miR-30a inhibitors, and pRc/CMV2-beclin-1: treated with pRc/CMV2-beclin-1. *P<0.05 compared with Con group. (C) Evaluation of the influence of down-regulation of beclin-1 on autophagic vacuoles. Ang II: treated with 1 µmol/L Angiotensin II, Ang II+3-MA: treated with 1 µmol/L Angiotensin II and 10 mmol/L 3-MA, Ang II+siRNA: treated with beclin-1-specific siRNA and 1 µmol/L Angiotensin II, and Ang II+miR-30a mimics: treated with 1 µmol/L Angiotensin II and miR-30a mimics. *P<0.05 compared with Ang II group. Data are presented as means ± SEM.