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. 2013 Jan 9;8(1):e53950. doi: 10.1371/journal.pone.0053950

Figure 8. Beclin-1 is a target gene of miR-30a.

Figure 8

(A) Sequence alignment between miR-30a and the 3′-UTR of beclin-1 in several species. (B) The schematic diagram of the plasmid of pGL3-Beclin-1 3′-UTR-Wild Type and pGL3- Beclin-1 3′-UTR-Mutant. The wild-type and mutated sequences of the target site of miR-30a on the 3′-UTR of Beclin-1 were shown. (C) Analysis of the relative luciferase activity in cardiomyocytes cotransfected with pGL3-Beclin-1 3′-UTR-Wild Type or pGL3-Beclin-1 3′-UTR-Mutant and miR-30a mimics, miR-30a inhibitors and negative control.*P<0.05 compared with NC group. (D) Evaluation of the influence of miR-30a on mRNA level of beclin-1 in cardiomyocytes. *P<0.05 compared with NC group. (E) Evaluation of the influence of miR-30a on expression of beclin-1 and LC3II/LC3I proteins in cardiomyocytes. *P<0.05 compared with NC group.