Figure 3.

The mutants’ interactions with PIC and INT-407 cells. The wildtype and mutant strains were added to the monolayers to achieve a multiplicity of infection (MOI) of 1:100, respectively. (a) Adherence and invasion of PIC. (b) Adherence, invasion, and intracellular survival in INT-407. Statistically significant (P < 0.05) differences are highlighted with * and indicate comparisons with the wildtype. The experiment was repeated three times independently and samples were tested in duplicate per experiment. Data are presented as mean ± standard error.