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. 2012 Nov 5;12:203. doi: 10.1186/1471-2229-12-203

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Copyright ©2012 De-la-Peña et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Immunoblot analyses of histone modification in Agave fourcroydes clones P20, P21 and P159 and A. angustifolia clone BM26. Analyses were carried out from plantlets cultured in multiplication medium (T0), semisolid growth medium in Magenta boxes (M), temporary immersion in modular Bioreactors (B), soil previously cultured in M (SM) and soil previously cultured in B (SB). Similar amounts of histone H3 were loaded as confirmed with an H3-specific antibody detecting the unmodified C-terminal part of H3.