Figure 6.

Effects of in vitro conditions upon the histone H3-methylation patterns of Agave fourcroydes clone P20 and A. angustifolia clone BM26 using Chromatin ImmunoPrecipitation (ChIP). Samples were collected from plants cultured in multiplication medium (T0), semisolid growth medium in Magenta boxes (M), temporary immersion in modular bioreactors (B), soil previously cultured in M (SM) and soil previously cultured in B (SB). The plant samples were examined for the Histone H3-tail methylation patterns the AtqKNOX1 and AtqKNOX2 genes. Input (input DNA), tenfold-diluted samples were used as templates for the input lanes. Negative controls (−Ab) with no antibody samples were treated in the same way as immunoprecipitated chromatins with H3K4me3 and H3K9me2. Amplified UBIQUITIN11 with specific primers was used as a control for the quality of the samples.