Table 1.
Oligo sequences and accession numbers
| Sequence (5’ to 3’) | Strand | Accession | |
|---|---|---|---|
|
siRNA oligos |
|
|
|
| FGFR3 |
CAGGUGUCCUUGGAGUCCAGUUCAU |
Sense |
AY737276 |
| |
AUGAACUGGACUCCAAGGACACCUG |
Anti-sense |
|
| FGFR2 |
GGGAAUAUACGUGCUUGGCGGGUAA |
Sense |
AJ320477 |
| |
UUACCCGCCAAGCACGUAUAUUCCC |
Anti-sense |
|
| Scrambled control (ScR) |
CAGUGCCUUGGGACUACUGUUGCAU |
Sense |
Invitrogen |
| |
AUGCAACAGUAGUCCCAAGGCACUG |
Anti-sense |
|
|
Real-Time PCR primers |
|
|
|
| FGFR3 |
CGCAGGACACCAGGTCTTTG |
Forward |
NM 174318.3 |
| |
cggcTACTCCTTCGACACCTGCG |
Reverse |
|
| TERT |
CGCTCCTTCCTGCTCTGCTC |
Forward |
NM 001046242.1 |
| |
cggacGATGGTTTCCACGAGTGTC |
Reverse |
|
| TR |
GCAGACTGGATGGTGGATGG |
Forward |
NR 001576.1 |
| |
cggtaCGCTGTGCTTTTGGTTACG |
Reverse |
|
| Kanamycin resistance - Exogenous Standard |
AAGCCCACTGCAAGCTACCTG |
Forward |
Invitrogen PCRIII vector based amplicon |
| CGTTTTGGCTATCTGGACAAGGGAAA | Reverse |