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Cells overexpressing TbCof with an HA3-tag at the C-terminus were treated with tetracycline (1 µg/ml) for 2 days. (A) The overexpressed TbCof-HA3 examined by western blot using an HA probe. (B) Cells overexpressing TbCof-HA3 stained with an HA probe and DAPI, and examined with a fluorescence microscope. TbCof is localized to the cytoplasm throughout the cell cycle.
