| P-gp |
Sense: 5′-CCCATCATTGCAATAGCAGG-3′
|
94°C for 30 s, 50°C for 30 s and 72°C for 30 s (35 cycles) |
|
Antisense: 5′-GTTCAAACTTCTGCTCCTGA-3′
|
|
| Ezrin |
Sense:5′-GAATACACTGCCAAGATTGC-3′
|
94°C for 30 s, 56°C for 45 s and 72°C for 45 s (35 cycles) |
|
Antisense: 5′-CTCATGTTCTCGTTGTGGAT-3′
|
|
| Radixin |
Sense: 5′-GCTAGGTGTTGATGCTTTGG-3′
|
94°C for 30 s, 52°C for 30 s and 72°C for 40 s (35 cycles) |
|
Antisense: 5′-GACGTTCCATTAGCTCTTCC-3′
|
|
| Moesin |
Sense: 5′- AGCCTTGGCATCTAGAGCTTGATGC -3′
|
94°C for 45 s,58°C for 45 s and 72°C for 1 min (35 cycles) |
|
Antisense: 5′- GGACTGAACCCTGGGGAGAAGACA-3′
|
|
| GAPDH |
Sense: 5′-CCATGGAGAAGGCTGGGG-3′
|
94°C for 30 s, 65°C for 30 s and 72°C for 40 s (35 cycles) |
|
Antisense: 5′-CAAAGTTGTCATGGATGACC-3′
|
|
