Figure 5. Evaluation of LC3-II and SQSTM1 in the presence of Baf A₁ after induction and/or inhibition of autophagy. (A) Corneal fibroblasts were cultured in the presence of Baf A₁ (0.1–0.2 μM) for the indicated times and then subjected to immunoblot analysis using anti-LC3, anti-SQSTM1, anti-TGFBI and anti-ACTB antibody. (B and C) Quantification of the LC3-II, SQSTM1 and TGFBI levels in (A) from three independent experiments using image analysis software. Error bars: SD from three independent experiments. *p < 0.05. Baf A₁ (0.1–0.2 μM) were added to the medium where indicated.