Figure 2. Depletion or inhibition of AURKA downregulated SQSTM1 through autophagy. (A) BT-549 cells were treated with 100 nM AURKA siRNA and NC siRNA for 24 h respectively, and then treated with the autophagy inhibitor bafilomycin A₁ (BAF, 200 nM) for 10 h. Cell lysates were subjected to western blot analysis with the indicated antibodies. (B) BT-549 cells exposed to MAP1LC3 siRNA. Twenty-four hours after siRNA transfections, cells were transfected with AURKA siRNA for an additional 24 h. Cell lysates were analyzed by western blot. (C) BT-549 cells were pretreated with 200 nM BAF for 1 h, and then treated with 10 nM VX-680 (VX) for an additional 24 h. Cell lysates were subjected to western blot analysis with the indicated antibodies. (D) BT-549 cells exposed to MAP1LC3 siRNA for 24 h, and cells were treated with 10 nM VX-680 (VX) for an additional 24 h. Cell lysates were assayed by western blot analysis similarly.