Figure 4. Overexpression of AURKA inhibited autophagy under conditions of nutrient deprivation. (A–C) Control (C) and overexpressed AURKA (A) MCF-10A, SK-BR-3 and BT-549 cells were subjected to HBSS starvation for the indicated time intervals. Pepstatin A (P, 10 μg/ml) and E64-d (E, 10 μg/ml) were added directly to the culture 4 h before lysis (left panel). Cells were not treated with P and E before lysis (shown in right panel). Cell lysates were subjected to western blot analysis. Densitometry analysis of LC3-II levels relative to ACTB was performed using Image J software.