Figure 6. Inhibition of autophagy potentiated cytotoxicity by depletion or inhibition of AURKA in breast cancer cells. (A and B) BT-549 and SK-BR-3 cells were transfected with 100 nM NC siRNA, MAP1LC3 siRNA and ATG5 siRNA respectively. Twenty-four hours later, they were treated with increasing doses of VX-680 for 24 h, cell viability was measured using MTT assay. (C and D) BT-549 and SK-BR-3 cells were pretreated with 100 μM chloroquine (CQ) and 200 nM bafilomycin A₁ (BAF) for 1 h, and then cells were treated with increasing dose VX-680 for additional 24 h, cell viability was measured using MTT assay. (E) SK-BR-3 cells were transfected with 100nM NC siRNA and AURKA siRNA respectively. Twenty-four hours later, cells were treated with 100 μM CQ and 200 nM BAF for 24 h, and then cell viability was measured using the MTT assay. Data are the means of triplicate experiments. *p < 0.05; **p < 0.01.