Figure 2. Transmission of TYLCV from viruliferous females or males of B. tabaci B biotype to non-viruliferous tomato plant. Relative gene expressions of TYLCV in tomato leaf were measured using a qRT-PCR after viruliferous whiteflies fed on the non-viruliferous tomato plants for 7, 14, 21, and 28 day. 50 viruliferous females or males were used to infect a tomato plant with non-TYLCV, during a 48 h IAP, following a 24 h AAP in order to obtain identical virus content whiteflies. Three biological replicates were used and 50 whiteflies per plant. Significant differences were denoted based on two-sample unpaired t-test.