Table 2.
Validation gene set, genes with significant changes in one or more of the resistant cell lines according to microarray analysis
| Cell line | Gene designation | Protein name | General function | Fold change | P value for fold change | Fluorescence value resistant line | Fluorescence value parent line |
|---|---|---|---|---|---|---|---|
|
A2780CBN |
AKR1C3 |
Aldo-keto reductase family 1 member C3 |
Catalyzes the conversion of aldehydes and ketones to alcohols |
−3.07 |
4.94 x 10-3 |
18.75 |
56.25 |
| |
ANXA1 |
Annexin A1 |
Calcium dependent phospholipid binding protein |
−104.15 |
1.38 x 10-4 |
96.50 |
9992.50 |
| |
CDH7 |
Cadherin 7 |
Cell to cell adhesion glycoprotein |
2.08 |
1.29 x 10-4 |
67.25 |
32.75 |
| |
GCLC |
Glutamate-cysteine ligase catalytic subunit |
Rate limiting enzyme of glutathione synthesis |
8.50 |
5.85 x 10-5 |
6907.25 |
815.00 |
| |
GSTO1 |
Glutathione S-transferase omega 1 |
Stress response protein, catalyzes addition of glutathione to toxic substrates |
2.38 |
1.07 x 10-3 |
23166.50 |
9726.50 |
| |
MT2A |
Metallothionein 2A |
Heavy metal binding protein |
3.69 |
4.15 x 10-6 |
4899.50 |
1266.00 |
| |
PARP9 |
Poly(ADP)-ribose polymerase family, member 9 |
Catalyzes addition of ADP-ribose moieties to substrate proteins |
9.40 |
1.95 x 10-3 |
2523.25 |
275.25 |
|
A2780DXL |
CYP1B1 |
Cytochrome P450 family 1, subfamily B, polypeptide 1 |
Phase 1 enzyme in drug metabolism |
−37.77 |
2.00 x 10-8 |
491.50 |
14858.38 |
| |
LGI1 |
Leucine-rich glioma inactivated 1 |
Metastasis regulator |
175.85 |
1.03 x 10-9 |
5449.25 |
30.00 |
| |
ABCB1 |
ATP-binding cassette transport subfamily B, member 1 |
Multidrug transporter (MDR/TAP family) |
33.62 |
8.22 x 10-11 |
21065.40 |
649.60 |
| |
ABCB4 |
ATP-binding cassette transport subfamily B, member 4 |
Multidrug transporter (MDR/TAP family) |
141.27 |
5.88 x 10-10 |
11876.75 |
84.63 |
|
A2780CBNDXL |
ABCB1 |
ATP-binding cassette transport subfamily B, member 1 |
Multidrug transporter (MDR/TAP family) |
35.28 |
2.43E-03 |
474.25 |
13.00 |
| |
ABCB4 |
ATP-binding cassette transport subfamily B, member 4 |
Multidrug transporter (MDR/TAP family) |
22.70 |
1.89x10-03 |
108.75 |
5.00 |
| |
AKR1C3 |
Aldo-keto reductase family 1 member C3 |
Catalyzes the conversion of aldehydes and ketones to alcohols |
7.30 |
2.62 x 10-3 |
400.75 |
55.75 |
| |
FLRT3 |
Fibronectin Leucine-rich Repeat Transmembrane protein 3 |
Helps regulate cadherin mediated cell adhesion and cell morphogenesis |
−115.40 |
1.79 x 10-3 |
39.50 |
4423.25 |
| |
GSTO2 |
Glutathione S-transferase 2 |
Stress response protein, catalyzes addition of glutathione to toxic substrates |
5.34 |
2.68 x 10-4 |
2133.25 |
400.00 |
| |
CDH7 |
Cadherin 7 |
Cell to cell adhesion glycoprotein |
68.82 |
9.24 x 10-4 |
5223.75 |
75.70 |
| CDH11 | Cadherin 11 | Cell to cell adhesion glycoprotein | 1022.24 | 1.57 x 10-4 | 10708.75 | 10.50 |