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. 2013 Jan 9;8(1):e52687. doi: 10.1371/journal.pone.0052687

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© 2013 Hu et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The tree was constructed using the neighbor-joining method in Mega software version 4.0. The scale at the bottom of the tree indicates the number of nucleotide substitutions per site. Horizontal branch lengths are proportional to the genetic distance, while vertical branch lengths have no significance. The numbers above each branch represent the percentage of 1000 bootstrap replicate support. The tree was rooted using an outgroup sequence of Murray Valley encephalitis virus (MVEV). Newly isolated JEV strains in Wuhan are labeled with black diamonds on the left and belong to genotype 1. The older strain belonging to genotype 3 is labeled with a black square.