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. 2012 Nov 20;52(1):64–71. doi: 10.3164/jcbn.12-60

Fig. 9.

Fig. 9

A representative result of CD4 and IFN-γ dual staining and flow cytometry. After 2 h stimulation with GEM, PBMCs were incubated with PHA for 48 h. CD4 and intracellular staining for IFN-γ were evaluated by flow cytometry.