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Absorption spectra of folded FeIII–Cyt c′ and FeII–Cyt c′ (bold lines), and unfolded FeIII–Cyt c′ and FeII–Cyt c′ (dashed lines). (Inset) GuHCl titration curves generated from CD, fluorescence, and Soret absorption data (χu is the unfolded-protein fraction).
