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Effect of different polymer concentrations on the protein encapsulation efficiency in PLGA nanopheres.*
| Batch | Concentration of PLGA 50:50 in ACN (mg/ml) | Encapsulation efficiency (%) |
|
|---|---|---|---|
| Lysozyme | α-Chymotrypsin | ||
| 1 | 38 | 26 ± 6 | 11 ± 4 |
| 2 | 63 | 45 ± 12 | 24 ± 4 |
| 3 | 95 | 68 ± 7 | 30 ± 1 |
| 4 | 190 | 94 ± 5 | 23 ± 3 |
Protein concentration: 25 mg/ml; volume ratio of water to organic solvent: 1:4; polymer mass: 380 mg; volume ratio of dispersing-to-diffusing phase: 1:40; theoretical protein loading: 5%.
