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Effect of the polymer concentration on α-chymotrypsin encapsulation efficiency in PLGA nanospheres.*
| Batch | Concentration of PLGA 50:50 in ACN (mg/ml) | Encapsulation efficiency (%) |
|---|---|---|
| 1 | 28.5 | 74 ± 4 |
| 2 | 47.5 | 49 ± 9 |
| 3 | 71.25 | 48 ± 4 |
| 4 | 142.5 | 38 ± 18 |
Protein concentration: 15 mg/ml; volume ratio between water and organic solvent: 1:4; polymer mass: 285 mg; volume ratio of dispersing-to-diffusing phase: 1:40 ml; theoretical protein loading: 5%.
