Table 1.

Dynamic phosphmetabolomic platform*

Metabolomics →	Dynamic phosphometabolomics (stable isotope 18O-labeling) →	Clinical metabolomics and 18O phosphometabolomics
Metabolome, static metabolic profile. Technologies: Tissue, cell and body fluid samples GC/MS LC/MS 1H NMR 31P NMR	Phosphometabolite turnover rates, metabolic flux distribution analysis in phosphotransfer networks and dynamics of metabolic cycles. Technologies: 18O-labeling of tissue, cell and whole blood samples 18O-assisted GC/MS 18O-assisted LC/MS 18O-assisted 31P NMR Model-based flux analysis	Metabolomics + Dynamic phosphometabolomics = system and network approach, disease mechanisms, more accurate disease prediction, diagnosis, treatment choices and efficacy. Technologies: 18O-labeling of tissue, cell and whole blood samples 18O-assisted GC/MS 18O-assisted LC/MS Correlation and predictive algorithms

*Abbreviations: GC – gas chromatography; MS – mass spectrometry; LC – liquid chromatography; NMR – nuclear magnetic resonance.