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In situ hybridization analysis for leptin mRNA expression in carotid atherosclerotic plaques. A, Human carotid atherosclerotic lesion. Leptin mRNA expression in cells surrounding cholesterol clefts, presumably macrophages. B, Spindle-shaped cells, presumably SMCs, exhibit positive staining for leptin mRNA. C and D, Human adipose tissue, using antisense as positive control (C), or sense probe as negative control (D). Scale bars = 50 μm.
