Figure 10.

Arterial retention of chylomicron remnants in MetS rats treated with ezetimibe (EZ) alone or in combination with simvastatin (EZ+SV). JCR:LA-cp rats were fed a lipid-balanced diet for 8 weeks. MetS JCR:LA-cp rats were randomized to a lipid-balanced diet with or without EZ alone (EZ; 0.01% w/w) or in combination with SV (EZ+SV; 0.01+0.01% w/w) for 8 weeks. Images of the arterial retention of intestinal-derived remnant lipoproteins in all treatment groups are shown in A through H. A through D, Fluorescence associated with Cy5-labeled intestinal-derived remnant lipoproteins (red). E through H, Composite overlay of fluorescence associated with remnant lipoproteins (red) and vessel morphology (green). Tissue sections were fixed and fluorescence visualized by confocal laser scanning microscopy (magnifications ×10 and ×60). I indicates intima; M, media; AV, adventitia; and MetS, metabolic syndrome. These data were subsequently quantified (Methods) and presented as the arterial retention of apoB48 (mg/µm³ tissue (1×10⁻¹²) (panel I); and remnants-derived cholesterol (mg/µm³ tissue (1×10⁻¹²) (panel J); (n=3, incorporating both carotids, 2 to 3 sections from each vessel, complete cross sections at ×10 magnification from each rat). Different letter subscripts (a through d) all indicate a significant difference of P<0.05 between the groups according to 1-way ANOVA. Values with the same letter subscript are not statistically different.