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. 2012 Oct 25;1(5):e003434. doi: 10.1161/JAHA.112.003434

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© 2012 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley-Blackwell.

This is an Open Access article under the terms of the Creative Commons Attribution Noncommercial License, which permits use, distribution, and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Figure 8. — Total aortic sulfated proteoglycans (A) and characterization of total sulfated GAGs isolated from lean and MetS rats (B). Total sulfated GAGs were isolated from lean and MetS JCR:LA-cp rat aortae at 12 weeks of age. Intact and enzymatically digested (chondroitin ABC lyase) samples were characterized on a SDS-PAGE gel by staining the gel with 1,9-dimethyl methyleneblue (DMMB) dye, which stains the sulfated groups. DMMB-stained gel (B) shows GAGs isolated from lean (lane 2) and MetS (lane 4) JCR:LA-cp rat aortae. Lanes (1) and (3) show chondroitin ABC lyase–digested samples of lean and MetS total sulfated GAGs, respectively. MetS indicates metabolic syndrome.