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. 2012 Oct 25;1(5):e004093. doi: 10.1161/JAHA.112.004093

Figure 7.

Figure 7.

H2S stimulates nitrite reduction to NO in hypoxic endothelial cells. A, Release of NO after administration of 50 μmol/L H2S with a chemiluminescent NO analyzer under normoxic (21% O2) and hypoxic (1% O2) conditions. B, Release of NO after administration of 50 μmol/L H2S after the addition of cPTIO or paraformaldehyde. C, Release of NO after administration of 50 μmol/L H2S after treatment of cells with sulfanilamide or febuxostat. D, Amounts of released NO after administration of different doses of H2S in the presence of paraformaldehyde (3%), NEM (10 μmol/L), sulfanilamide (1 mmol/L), febuxostat (10 nmol/L), cPTIO (200 μmol/L), or L‐NAME (300 μmol/L). E through G, Interaction between H2S and nitrite followed by amount of NO generation in the presence or absence of recombinant XO (0.0025 U, 0.005 U, and 0.01 U/mg). n=6 replicates performed in triplicate. *P<0.01 compared to PBS control data.