WNT3 is a KDM6A/KDM6B target contributing to early endoderm differentiation. (A)
RT-qPCR analysis of the expression of 7 H3K27me3-enriched WNT ligand genes during
definitive endoderm differentiation. WNT2, WNT2B, WNT5B,
WNT6, WNT9B, WNT10A and WNT16 are not detectable.
Expression levels are related to day 0. Data presented is the average of three
independent experiments with error bars. (B) RT-qPCR analysis demonstrates
downregulation of WNT3 expression upon KDM6A or KDM6B knockdown. (C)
ChIP analysis demonstrates increased H3K27me3 level, but not H3K4me3, at the
WNT3 gene promoter upon KDM6A or KDM6B knockdown. The analysis was performed
at the beginning of differentiation. (D) Activin A-induced endoderm
differentiation is compromised by WNT3 knockdown, which can be rescued by the addition
of the WNT agonist Wnt3a. The differentiation efficiency is measured by quantification
of CD184-positive cell population after 4-day activin A-based endoderm
differentiation.