Figure 2. .
Proliferation of PKH26-stained human PBMC incubated 3 days with (a) 1 μg/well aqueous TGN1412 vs (b) 1 μg/well immobilized TGN1412. Lymphocyte proliferation appears as bands of cells with decreasing PKH26 fluorescence as dye is divided equally between daughter cells and increased forward scatter (FSC) corresponding to increased size due to blasting. No proliferation is observed with aqueous TGN1412 (a) compared to a strong proliferative response with immobilized TGN1412 (b). Low power light microscope images of 2 × 105 human PBMC in tissue culture after 3 days culture with (c) 1 μg/well aqueous TGN1412 vs (d) 1 μg/well immobilized TGN1412.