Figure 3. .
Cytokine release from human PBMCs stimulated in vitro for 24, 48, and 72 h with 1 µg/well immobilized isotype-matched control (IgG4κ), Rituximab (IgG1), Campath-1H (IgG1), and TGN1412 (IgG4κ). The lectin PHA at 10 µg/ml was used as mitogen control. Cytokine release into culture supernatants was measured by ELISA. (a) TNFα, (b) IL-8, (c) IFNγ, and (d) IL-2 release are expressed in pg/ml. Donors responses shown (n = 9). * Denotes p < 0.05 (one-way analysis of variance with bonferroni correction).