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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Cancer Res. 2012 May 2;72(13):3260–3269. doi: 10.1158/0008-5472.CAN-11-4141

Figure 2. NF-κB target genes are upregulated by oncogenic PI3K mutations.

Figure 2

MCF10A cells stably expressing GFP, PIK3CA WT, E545K, or H1047R were starved for 24h and treated with DMSO or 2μM BAY-65-1942 for 4h. A) Cells were lysed and evaluated by immunoblot. B) Microarray analysis was performed. Genes which exhibit both increased expression in PIK3CA-transformed cells and decreased expression following IKK inhibition are shown. C–D) Real-time RT-PCR was used to verify expression changes for IL-6 and CXCL1. E–F) ELISA analysis shows increased secretion of IL-6 and CXCL1 by cells expressing the oncogenic PI3K mutations following 24h GF-deprivation.