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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Cancer Res. 2012 May 2;72(13):3260–3269. doi: 10.1158/0008-5472.CAN-11-4141

Figure 4. Sustained PI3K inhibition decreases expression of NF-κB target genes in cells expressing oncogenic PI3K mutations.

Figure 4

A) MCF10A cells expressing PIK3CA H1047R were treated for 4h or 24h with 10μM LY294002 and analyzed by immunoblot. B) RNA samples from cells treated as in A) were evaluated by microarray. C) Venn diagram depicting changes in NF-κB-dependent gene expression following 4h or 24h of LY294002 treatment. C–D) MCF10A cells expressing PIK3CA E545K were treated for 4h or 24h with 10μM LY294002 and analyzed by real-time RT-PCR to evaluate expression of C) IL-6 or D) CXCL1.